The genomic information and transcriptome sequences of Batrachuperus tibetanus are rarely reported at present.In this study,the transcripts of this species were sequenced and assembled using Illumina HiSeq platform.A total of 36 252 unigenes were obtained through sequencing and assembling,with an average length of 937 bp and a N50 of 1 549 bp.Additionally,GC content,length distribution and expression level of unigenes were assessed.The result showed that the sequencing data was of high quality.Blasted against the Swiss-prot,Nr,Pfam,KEGG,KOG and GO,a total of 16 465,18 749,13 983,10 607,15 704 and 14 242 unigenes were annotated,respectively.According to the functional annotation results,17 472 coding sequence (CDS) were detected.A total of 2 779 microsatellite markers (SSR) and 3 100 single nucleotide polymorphisms (SNP) were identified.KEGG pathway analysis showed that 10 607 unigenes were annotated,and it was divided into 257 categories according to the different pathways.Among all the pathways,the number of unigenes involved in signal transduction pathway exhibited the largest proportion.In this study,the obtained transcriptome sequences provided rich data resources for further understanding of genetic diversity analysis,molecular marker development,population resource evaluation and conservation,as well as functional gene cloning of the B.tibetanus.

From April to December in 2021,68 typical farms and 265 culture questionnaires of diamond shrimp(Penaeus monodon)were investigated in Zhangzhou City,Fujian Province.The results showed that there were mild climate,long sunshine time and long coastline in Zhangzhou,which was suitable for the shrimp culture.The mainly diamond shrimp culture mode in Zhangzhou were using soil pond and higher place pond,where were mainly distributed in Longhai District,Zhangpu County,Dongshan County and Zhao’an County,respectively.There were 2-3 times harvests a year which the stocking density,survival rate,feed coefficient,output and profit were(1.50-4.50)×10⁵ ind/hm²,73.90%,1.63,(0.38-0.98)×10⁴ kg/hm² and RMB(1.20-12.75)×10⁴ yuan/hm² in 3-10 salinity mixed by groundwater and bittern in soil pond,respectively.There were 2-3 times harvests a year too,which the stocking density,survival rate,feed coefficient,output and profit were(1.50-6.00)×10⁶ ind/hm²,74.58%,1.84,(0.83-10.20)×10⁴ kg/hm² and RMB(3.53-11.09)×10⁵ yuan/hm² using 20-30 salinity natural seawater in higher place pond,respectively.The production of diamond shrimp larvae was increased year by year in Zhangzhou which the increase amount were 7.76% to 39.41% from 2017 to 2020.There was a slightly decline by 32.46% in 2021.At present,the basis and age of the employees of diamond shrimp farmers were poor and old in Zhangzhou,respectively.There was a single specie for selection.The stocking density of higher place pond had been blindly increased,and the aquaculture tailwater was difficult to be treated.The fine management was employed to improve the breeding efficiency,and the "white + black(Litopenaeus vannamei + P.monodon)" healthy breeding mode was popularized for promoting the sustainable and healthy development of diamond shrimp culture.